Molecular Detection Core
(Core B)

Contact:
Cecelia Latham  

MDC Director:  
Dr. Kevin Roth

Additional Core Sites

Core A
Behavioral Assessment Core

Core A Director:  
Dr. Michael Wyss
 
Contact:
Dr. Thomas Van Groen

Core C
Protein Interaction Core
 
Core C Director: 
Dr. Bill Britt 

Contact:
Mary Ballestas, PhD.

Home

Molecular Detection Core Resources
and Protocols

The MDC assists Neuroscience researchers in developing new protocols and trains UAB staff and students in the production of high quality, reproducible, molecular localization results on their cell and tissue samples. Assistance is available to determine optimal fixation conditions, tissue processing techniques and experimental design for the detection of nervous system-associated molecules of interest. 

Contact Cecelia Latham at 996-6556 or 934-5474 for more information related to MDC activities.

Resources: 

• UAB Neurosciences Blue Print Core Center

• Antibodies

• Tissue processing facilities

• Automated staining resources

• Multi labeling

• Detection methods: conjugated secondaries, TSA, TSA PLUS,
  Q-dots, chromogenic 

Primary Antibody Resources:

• Santa Cruz Biotechnology, Inc.

• SIGMA-ALDRICH

• Cell Signaling Technology

• Dako

• BD Biosciences

• Abgent

• Millipore (includes Serologicals’ Chemicon, Upstate and Linco Millipore’s Bioscience Division)

• Invitrogen (includes Zymed antibodies and reagents)

• Leica-microsystems (includes Novocastra labs)

Secondary Antibody Resources

• Jackson ImmunoResearch Labortories, Inc. (And many of the primary antibody suppliers listed above.)

• Vector Labs (Impress HRP polymer Horse secondaries and ABC reagents)

• Zymed/Invitrogen (SuperPicture Goat anti-Rabbit-HRP polymer SKU# 87-9263)

Tissue Processing Facilities 

• UAB-Animal Resources Comparative Pathology Laboratory 
  Phone: 934-7889 for additional information. 

• UAB-Comprehensive Cancer Center Tissue Procurement Core Facility Location: ZRB-449
  Phone: 934-6071 for price schedule and services. 

• Alabama Neuroscience Blueprint Center Core-C (Histology)

• Off campus commercial research histology services: 
  AML Labs, Inc. 
  Contact: 877-219-5183 or service@amllabs/cp 

How-to of Tissue Processing:

• Molecular Detection Core Tissue Processing Protocol

• UAB Tissue Submission Guide

• Unit 14.1 (Fixation, Embedding, and Sectioning of Tissues, Embryos, and Single Cells) in Current Protocols in Molecular Biology (CPMB): Copyright ©2005 by John Wiley & Sons, Inc. All Rights Reserved

Protocols and Reagents:

• IHC protocols
  Immunohistochemical Detection with Quantum Dots (PDF 3mb)

• ISH protocols

• Reagents (PDF)

For General Information on IHC and ISH:

• For background information on In Situ Hybridization and Immunohistochemistry see Chapter 14 in Current Protocols in Molecular Biology (CPMB):Copyright ©2005 by John Wiley & Sons, Inc. All Rights Reserved.
  

• The Allen Brain Institute protocols for RNA ISH 

Tyramide Signal Amplification Strategies for Fluorescence Labeling

• Click here for short course in PDF format.
  Roth, K. A. (2008) Tyramide Signal Amplification Strategies for Fluorescence Labeling In: Seeing Is Believing: Antibodies and How To use Them. (Hoffman G, ed) pp. [19-26]. Washington, DC: Society for Neuroscience.
  

• Tyramide Signal Amplification

TUNEL Protocol Outline

Click here for a PDF of this protocol.
The following links are related to this protocol and are also contained in the PDF:

Apoptag